Modeling The Migration And Proliferation Of Endothelial Cells with Changing Shape and Size

Aparna Ambani (1), K. Zygourakis (2) and P. Markenscoff (1)

1 Dept. of Electrical and Computer Engineering
University of Houston.

2 Dept. of Bioengineeringand Chemical Engineering
Rice University, Houston, TX.

 

ABSTRACT

Due to the multitude of parameters affecting the dynamics of cellular systems, the development of engineered tissues will be greatly facilitated by mathematical models that can predict cellular movement and tissue growth. This study focuses on analyzing the opposing effects of contact inhibition and migration on the proliferation rates of endothelial cells (EC). A new model based on the concept of cellular automata is developed to accurately describe the dynamics of migrating and proliferating EC. Each EC can now occupy several adjacent sites on the computational grid and its shape varies with time as the cell changes directions, collides with other cells and divides. Changes in the direction of cell movement (caused by cell-cell collisions or intracellular events) involve the formation of a leading lamella pointing in the new direction. The new model is a significant extension of earlier models developed by our group. Simulation results show that cell proliferation rates are significantly affected by the speed of locomotion, the persistence of cell movement and the elongation of migrating cells. Also, the cell shape and cell density affect the population-average speed of migration. Model predictions agree well with the results of cell growth experiments.


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